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| Stemfect™ DNA Plasmid Transfection Polymer |
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| Description |
| Stemfect™ DNA Plasmid Transfection Polymer is a novel polymer specifically designed to transfect stem cells. It enables 2-5× improved transfection efficiency on stem cells compared to Lipofectamine™ 20001. Stemfect™ Polymer is a beta-amino ester polymer that provides unique structural features that aid in plasmid complexation, cellular entry, and intracellular distribution. In addition, this polymer can be used in media containing serum and antibiotics. Stemfect™ Polymer was developed in the lab of Dr. Dan Anderson at MIT. A major focus in Dr Anderson's lab is the study and development of polymers to deliver drugs, particularly genetically engineered proteins, DNA and RNAi, continuously at controlled rates for prolonged periods of time. |
| Disclaimer |
| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
| Specifications and technical documents |
| Specification SheetsMaterial Safety Data SheetsProtocols
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| Figure 1 |
| Stemfect Kit. |
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| Details |
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| Products |
| Stemfect DNA Plasmid Transfection Polymer |
- 500 transfections in a 24-well plate - Stemgent Catalog # 00-0001 Components - Stemfect™ Polymer - Stemfect™ Buffer 130-095-533
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| References |
| 1. Green et al. (2008) Nano Lett. 8 (10): 3126–3130. |
| 2. Green et al. (2008) Acc. Chem. Res. 41 (6): 749–759. |
| 3. Green et al. (2007) Adv. Mat. 19: 2836–2842. |
| 4. Zugates et al. (2007) Bioconjug. Chem. 18: 1887–1896. |
| 5. Zugates et al. (2007) Mol. Ther. 15: 1306–1312. |
| 6. Peng et al. (2007) Prostate 67: 855–862. |
| 7. Green et al. (2006) Bioconjug. Chem. 17: 1162–1169. |
| 8. Anderson et al. (2005) Mol. Ther. 11: 426–434. |
| 9. Anderson et al. (2004) Proc Natl Acad Sci USA 101: 16028–16033. |
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