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| Stemfect™ 2.0-mESC DNA Transfection Reagent |
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| Description |
Stemfect™ 2.0-mESC DNA Transfection Reagent is a polymer-based transfection reagent specifically designed for in vitro DNA transfection of mouse embryonic stem (mES) cells. This reagent has demonstrated low cytotoxicity and 5X improved efficiency over leading transfection reagents in functional assays. The specificity of Stemfect™ 2.0-mESC DNA Transfection Reagent makes it a desirable alternative to broad spectrum reagents for transfecting mES cells. In addition, transfections with this reagent can be used in media containing serum and antibiotics.
Stemfect™ 2.0-mESC DNA Transfection Reagent belongs to a beta-amino ester polymer family, developed in the lab of Dr. Daniel Anderson at MIT, with unique structural features which aid in plasmid complexation, cellular entry, and intracellular distribution1-6. |
| Disclaimer |
| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
| Specifications and technical documents |
| Specification SheetsMaterial Safety Data SheetsProtocols
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| Figure 1 |
| Comparison of transfection efficiency on KH2 mES cells using Stemfect™ 2.0-mESC DNA Transfection Reagent, Reagent L, and Reagent F. |
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| Figure 2 |
| Mouse ES cells transfected with plasmid DNA encoding GFP using Stemfect™ 2.0-mESC DNA Transfection Reagent. |
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| Details |
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| Products |
| Stemfect 2.0-mESC DNA Transfection Reagent |
- 500 transfections in a 24-well plate
- Stemgent Catalog # 00-0010
Components
- Stemfect™ 2.0 Polymer
- Stemfect™ Buffer
130-095-535
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| References |
| 1. Yang et al. (2009) Gene Ther. 16: 533-546. |
| 2. Green et al. (2008) Nano Lett. 8: 3126–3130. |
| 3. Green et al. (2008) Acc. Chem. Res. 41: 749–759. |
| 4. Green et al. (2007) Adv. Mat. 19: 2836–2842. |
| 5. Peng et al. (2007) Prostate 67: 855–862. |
| 6. Anderson et al. (2004) Proc. Natl. Acad. Sci. USA 101: 16028–16033. |
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