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The MACSelect™ 4 System uses the truncated human CD4 molecule as a marker to select transfected cells. It can be used in virtually all CD4-negative cell lines and primary cells.*
pMACS 4-IRES.II enables the bicistronic expression of the gene-of-interest and the truncated CD4 molecule: with the IRES element (internal ribosomal entry site), the gene-of-interest is translated from the same mRNA as the selection marker CD4.
The pMACS 4.1 vector encoding the truncated CD4 surface marker is used for cotransfection in combination with a gene-of-interest expression vector.
The MACSelect 4 Transfected Cell Selection Kit provides the vectors, MACSelect MicroBeads, and fluorochrome-conjugated antibodies, including control vectors and antibodies required to set up and establish the MACSelect System. All kit components are also available separately.
* The CD4 molecule is sensitive to trypsin treatment and should, therefore, not be used in cell lines which need to be harvested with trypsin. |
| Disclaimer |
| The CMV promoter is covered under U.S. patents 5,168,062 and 5,385,839 and its use is permitted for research purposes only. Any other use of the CMV promoter requires a licence from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242. |
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| Figure 1 |
| The pMACS 4-IRES.II vector. |
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| * The BsmB I site is compatible with Hind III digested fragments. |
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| Figure 2 |
| The pMACS 4.1 vector. |
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| Figure 3 |
| Transfection of CHO cells with pMACS 4-IRES.II, magnetic labeling with MACSelect 4 MicroBeads and separation. FITC staining of transfected cells (CD4-FITC/MACSelect Control FITC), blue nuclear counterstain (Toto 3, Molecular Probes). |
| A: Less than 4% transfected cells after transfection |
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| B: More than 90% transfected cells after MACSelect enrichment |
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| Figure 4 |
| MACSelect transfected cell enrichment |
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| Favorites / Prices |
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| Details |
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| Products |
| MACSelect 4 - Transfected Cell Selection Kit |
- for 25 separations
Components
- 25 μg pMACS 4-IRES.II
- 25 μg pMACS 4.1
- 25 μg pMACS 14.1
- 0.25 mL MACSelect Control FITC Antibody
- 0.25 mL CD4-FITC, human
- 0.25 mL CD14-FITC, human
- 2 mL MACSelect 4 MicroBeads
Download datasheet
130-091-988
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| pMACS 4.1 |
Components
- 25 μg plasmid
Download datasheet
130-091-886
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| pMACS 4-IRES.II |
Components
- 25 μg plasmid
Download datasheet
130-091-888
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| MACSelect 4 MicroBeads |
- for 25 separations
Components
- 2 mL MACSelect 4 MicroBeads
Download datasheet
130-070-101
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| References |
| 1. Gaines et al. (1999) BioTechniques 26: 683-688. |
| 2. von Knethen et al. (1999) Macrophages. Mol. Biol. Cell 10: 361-372. |
| 3. Überall et al. (1999) J. Cell Biol. 144: 413–425. |
| 4. Kube et al. (1999) J. Virol. 73: 1630-1636. |
| 5. Szentirmay et al. (2003) J. Biol. Chem. 278: 37231-37240. |
| 6. Philipps et al. (2004) BioTechniques 36: 80-83. |
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