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| Stemgent® Mouse Primary Cells-NGFP3 |
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| Description |
The Stemgent® Primary iPS NGFP3 cell line is one of three induced pluripotent stem (iPS) cell lines that were generated by reprogramming Nanog-GFP; rtTA mouse embryonic fibroblasts (MEFs) using four Dox-inducible transcription factors (TFs) (Oct4, Sox2, Klf4, and c-Myc) delivered by lentiviral vectors1. The Nanog-GFP; rtTA MEFs2 used to generate these NGFP cell lines contain a constitutively expressed reverse tetracycline transactivator (rtTA) driven by the ROSA26 promoter (R26-M2rtTA) as well as GFP knocked-in to the endogenous Nanog locus (Nanog-GFP). This NGFP3 iPS cell line harbors both rtTA and GFP as well as the Dox inducible transcription factors [Oct4 (1 proviral integration), Sox2 (1 proviral integration), Klf4 (4 proviral integrations), and c-Myc (1 proviral integration)] inserted during lentiviral transduction. When cultured under standard mouse embryonic stem (ES) cell culture conditions, the morphology of NGFP3 iPS cells is identical to that of mouse ES cells. The cells express both GFP from the endogenous Nanog locus as well as the pluripotency marker SSEA-1. Additionally, they demonstrate strong endogenous alkaline phosphatase (AP) activity. Stemgent® Mouse Primary iPS cells-NGFP3 can be used to generate chimeric mice from which cells can be isolated for secondary reprogramming studies1,2. The simple addition of Doxycycline (Dox) to the growth medium used to propagate the isolated cell types from the chimeric mouse results in the upregulation in expression for the four transcription factors implicated in the reprogramming process.
The Stemgent® Mouse Primary iPS cells-NGFP3 were generated in the lab of Dr. Rudolf Jaenisch, M.D. at Whitehead Institute-MIT. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focused on ES and iPS cellular mechanisms and methodologies. |
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| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
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| Details |
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| Products |
| Stemgent Mouse Primary Cells-NGFP3 |
- Stemgent Catalog # 08-0011
Components
- 2 x 105 cells per vial
130-095-860
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| References |
| 1. Hanna, J. et al. (2008) Cell 133 (2): 250 |
| 2. Wernig, M. et al. (2008) Nat. Biotechnol. 26 (8): 916-924. |
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