Stemgent® Mouse Primary iPS Cells-NNeo ( #130-095-687 )

Stemgent® Mouse Primary iPS Cells-NNeo

Description

The Stemgent™ Mouse Primary iPS Cells-NNeo harbor the reverse tetracycline transactivator (rtTA) targeted to the ROSA26locus (ROSA26-M2rtTA), Oct4 cDNA under the control of tetracycline operator targeted to the type I collagen locus, a neomycin (neo) resistance gene in the endogenous Nanog locus, and the DOX-inducible transcription factors [Klf4 (3 proviral integrations), Sox2 (2 proviral integrations) and c-Myc (1 proviral integrations)] inserted during lentivirus transduction. Injection of these cells into blastocysts results in the generation of chimeric mice from which a variety of cell types (neural progenitor, adrenal gland, keratinocyte, muscle, and mouse embryonic fibroblast) can be isolated for secondary reprogramming studies¹. The simple addition of DOX to the growth medium used to propagate the isolated cell types from the chimeric mouse results in the upregulation in expression for the aforementioned 3 transcription factors implicated in the reprogramming process.

When cultured under standard mouse embryonic stem (ES) cell culture conditions, the morphology of Stemgent™ Mouse Primary iPS cells-NNeo is identical to that of mouse ES cells¹. The cells express pluripotency marker SSEA-1. Additionally, they demonstrate strong endogenous AP activity.

The Stemgent™ Mouse Primary iPS cells-NNeo were generated in the lab of Dr. Rudolf Jaenisch, M.D. at Whitehead Institute-MIT. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focuses on ES and iPS cellular mechanisms and methodologies.

Disclaimer

This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel.

Specifications and technical documents

Specification Sheets

08-0012 Specifications Sheet

Material Safety Data Sheets

08-0012 Material Safety Data Sheet

Protocols

Protocol: miPS Cell Culture