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| Description |
| Laminin is the main component of reconstituted basement membrane (RBM), isolated from the mouse Engelbreth-Holm-Swarm (EHS) tumor. This glycoprotein consists of three polypeptide chains (a, b, and g) containing sites that promote cell attachment and other biological activities. Embryonic stem (ES) cells express receptors for laminin that bind these sites in the native laminin molecule. The use of laminin as a growth matrix should be considered when the peptide growth factors present in RBM gels might confound an experiment. In pure form, laminin is sufficient to support excellent ES cell and iPS cell growth in the undifferentiated state in defined media with and without small molecule kinase inhibitors. In addition, laminin can serve as an alternative to mouse embryonic fibroblast (MEF) feeder cells for propagating pluripotent human cell lines, with or without serum, and can be used to support derivation and to promote the differentiation of lineage-restricted progenitor cells. |
| Disclaimer |
| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
| Specifications and technical documents |
| Specification SheetsMaterial Safety Data Sheets
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| Figure 1 |
| Stemgent® Laminin offers superior attachment of human and mouse embryonic stem cells. |
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| Figure 2 |
| Stemgent® Laminin is ultrapure with greater than 98% purity. |
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| Details |
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| Products |
| Stemgent Mouse Laminin |
- 1 mg
- Stemgent Catalog # 06-0002
130-095-602
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| References |
| 1. Ying et al. (2008) Nature 453: 519–523. |
| 2. Takahashi et al. (2007) Cell 131: 861–872. |
| 3. Dimos et al. (2008) Science 321: 1218–1221. |
| 4. Beck et al. (1990) FASEB J. 4: 148–160. |
| 5. Kleinman et al. (1988) Proc. Natl. Acad. Sci. USA. 85: 1282–1286. |
| 6. Mercurio and Shaw (1991) BioEssays 13: 469–473. |
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