Stemgent® mRNA Reprogramming Kit ( #130-097-191 )

Stemgent® mRNA Reprogramming Kit

Description

The Stemgent mRNA Reprogramming Kit contains three essential components for productive non-integrative reprogramming: the Stemgent mRNA Reprogramming Factors: OSKM, the Stemgent Pluriton™ Reprogramming Medium and B18R recombinant protein, carrier-free. Stemgent functionally validates all three components together. The in vitro transcribed mRNA’s encoding Oct4, Klf4, Sox2, c-Myc, Lin-28 factors and the nuclear GFP (nGFP) control, fluorescent reporter incorporate both pseudouridine and 5-methylcytidine-modified nucleotides, and when used in combination with the B18R recombinant protein minimize the cellular interferon response to single stranded RNA.^1,2 Repeated transfection of mRNA encoding Oct4, Klf4, Sox2, c-Myc and Lin-28 has been shown to reprogram human fibroblasts.³ nGFP mRNA is included for use as a positive control for RNA transfection during the cellular reprogramming process. In addition, the Pluriton Reprogramming Medium is a xeno-free medium included in the kit that was designed to support the robust and reproducible mRNA reprogramming of human fibroblasts.

Learn more about the Stemgent mRNA Reprogramming System.

Disclaimer

This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel.

Specifications and technical documents

Specification Sheets

00-0071 Specifications Sheet

Material Safety Data Sheets

mRNA Material Safety Data Sheet

Brochures

Brochure: Stemgent mRNA Reprogramming System

Manuals

User Manual: Stemgent mRNA Reprogramming System (updated January 2012)

Other Support Documents