|
|
 |
| Stemolecule™ Doxycycline hyclate |
|
| Description |
| Stemolecule™ Doxycycline hyclate (Dox) is a broad spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases in vivo. Tetracycline-controlled transcriptional activation is a method of inducible expression whereby transcription is reversibly turned on or off in the presence of tetracycline or one of its derivatives such as Dox1. Dox-inducible lentiviral reagents such as the Stemgent® iPSC Generation Dox Inducible Lentivirus products are used to induce the expression of virally transduced genes and generate induced pluripotent stem (iPS) cells from somatic cells (in a process referred to as reprogramming2,3) by adding Dox to the cell culture medium4-9. Stemolecule™ Doxycycline hyclate is the recommended inducer for all of the Stemgent® iPSC Generation Dox Inducible products. In the iPSC generation protocol, addition of Dox to the culture medium of transduced cells induces the reverse tetracycline transactivator (rtTA) to bind to the Tet response element and results in the transcriptional activation for each of the factors implicated in the reprogramming process. Conversely, Dox can be removed from the medium to suppress transcriptional activation of the reprogramming factors. |
| Disclaimer |
| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
| Specifications and technical documents |
| Specification SheetsMaterial Safety Data Sheets
|
| |
|
| Figure 1 |
| Stemolecule™ Doxycycline hyclate chemical structure. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| Stemolecule Doxycycline hyclate |
- 10 mg
- Stemgent Catalog # 04-0016
130-095-567
|
|
| References |
| 1. Bujard and Gossen (1992) Proc Natl Acad Sci 89: 5547–5551. |
| 2. Takahashi and Yamanaka (2006) Cell 126: 663–676. |
| 3. Yu et al. (2007) Science 318: 1917–1920. |
| 4. Wernig et al. (2007) Nature 448: 318–324. |
| 5. Brambrink et al. (2008) Cell Stem Cell 2: 151–159. |
| 6. Wernig et al. (2008) Nat Biotechnol 26 (8): 916–924. |
| 7. Hockemeyer et al. (2008) Cell Stem Cell 3 (3): 346–353. |
| 8. Welstead et al. (2008) J Vis Exp 7 (14): 734. |
| 9. Markoulaki et al. (2009) Nat Biotechnol 27 (2): 169–171. |
|
| MACS Stem Cell newsletter |
|
Stay current with important developments in stem cell research by subscribing to
Stem Cell Updates.
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
