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| Anti-Sca-1 MicroBead Kit (FITC) |
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| Description |
| The Anti-Sca-1 MicroBead Kit (FITC) was developed to isolate mouse hematopoietic stem cells and includes all reagents for fluorescent and indirect magnetic labeling of Sca-1+ cells, using Anti-Sca-1-FITC and Anti-FITC MicroBeads. Sca-1 (stem cell antigen-1) is a 18 kDa GPI-linked surface protein of the Ly-6 family (Ly-6A/E). The anti-Sca-1 monoclonal antibody recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). |
| Applications |
Hematopoietic stem cells in mouse bone marrow are defined as negative for lineage markers (lin–), and positive for Sca-1 and CD117 (c-kit). Long-term repopulating hematopoietic stem cells (LTR-HSCs) can be characterized by the expression of CD105+Sca-1+ 1,2. They can be enriched by using the CD105 MultiSort Kit (PE) in combination with the Anti-Sca-1 MicroBead Kit (FITC).
Sca-1+ cells from mouse bone marrow have further been shown to have nonhematopoietic differentiation potential (e.g. to hepatocytes and neural cells in vitro4). Additionally, Sca-1+ stem cells have been isolated from peripheral blood5, fetal6, and adult liver7 as well as heart8 and prostate tissue9.
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| Figure 1 |
| Separation of stem cells from mouse bone marrow after fluorescent and magnetic labeling using the Anti-Sca-1 MicroBead Kit (FITC) and a MiniMACS™ with two MS Columns. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
| A: Before separation |
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| B: Sca-1+ cells |
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| Products |
| Anti-Sca-1 MicroBead Kit (FITC), mouse |
for 109 total cells
Download data sheet
130-092-529
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| MACS References |
| 1. Chen, C.Z. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 15468–15473.[939] |
| 2. Chen, C.Z. et al. (2003) Immunity 19: 525–533.[601] |
| 3. Lagasse et al. (2000) Nat. Med. 6: 1229-1234.[206] |
| 4. Sanchez-Ramos et al. (2000) Exper. Neurol. 164: 247–256[7732] |
| 5. Yamada M. et al. (2004) J. Immunol. 172: 1266–1272.[863] |
| 6. Cherqui S. et al. (2006) Stem cells 24: 44–54.[10046] |
| 7. Petersen B.E. et al. (2003) Hepatology 37: 632–640.[11259] |
| 8. Matsuura K. et al. (2004) JBC 279: 11384–11391.[4144] |
| 9. Burger P.E. et al. (2005) Proc. Natl. Acad. Sci. USA 102: 7180–7185.[7082] |
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