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| Anti-Prominin-1 MicroBeads |
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| Description |
Anti-Prominin-1 MicroBeads have been developed for the isolation of Prominin-1+ mouse cells. Prominin-1 is a 115–120 kDa, 5-transmembrane glycoprotein with close structural similarity to its human ortholog, CD1331. Prominin-1 expression has been demonstrated on cells of different embryonic and adult tissues, including ES-derived committed and early progenitor cells2, the developing3 and postnatal4 central nervous system, kidney tubules3, and cells of the hematopoietic system3. In the developing nervous system prominin-1 is predominantly expressed on neuroepithelial cells and other epithelia. In the postnatal brain, prominin-1 expression has been found in areas of neurogenesis, such as the subventricular zone (SVZ). Prominin-1 is preferentially localized to plasma membrane protrusions of the apical membrane.5
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| Applications |
| Prominin-1+ cells selected by MACS® Technology can be used, e.g., for studying differentiation potential. Prominin-1+/Lineage– cells isolated from postnatal cerebellum were reported to be capable of differentiating into neurons, astrocytes and oligodendrocytes in vitro and in vivo.4 |
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| Figure 1 |
| Enrichment of Prominin-1+ cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS™ Separator, and MS Columns. |
| Mouse cerebellar cells before separation |
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| Prominin-1– cells |
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| Prominin-1+ cells |
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| References |
| 1. Weigmann et al. (1997) Proc. Natl. Acad. Sci. USA 94: 12425–12430. |
| 2. Kania et al. (2005) Stem Cells 23: 791–804. |
| 3. Corbeil et al. (2001) Traffic 2: 82–91. |
| 4. Lee et al. (2005) Nat. Neurosci. 8: 723–729. |
| 5. Marzesco et al. (2005) J. Cell Sci. 118: 2849–2858. |
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