| Description |
| The Lineage Cell Depletion Kit is a magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, monocytes/macrophages, granulocytes, and erythrocytes as well as their committed precursors. For depletion, cells are magnetically labeled with a cocktail of biotinylated antibodies against a panel of so-called lineage antigens (CD5, CD45R (B220), CD11b, Gr-1 (Ly-6G/C), 7-4, and Ter-119) and Anti-Biotin MicroBeads. |
| Applications |
| This labeling procedure leaves lineage-negative cells untouched; thus, allowing further magnetic separation of lineage-negative cells according to expression of markers such as CD1171,2 or Sca-13. Therefore, the Lineage Cell Depletion Kit has been used for the enrichment of both, hematopoietic stem cells (HSCs)4 and marrow stromal cells (MSCs) from bone marrow5. |
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