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| Overview |
GM-CSF stands for granulocyte macrophage colony-stimulating factor. Mouse GM-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Mouse GM-CSF can be used for a variety of applications, including:
- Induction of colony formation of granulocyte/macrophage progenitors in semi-solid medium.
- In vitro generation of DCs from bone marrow1 or the maturation of CD11c+ splenocytes.
- Generation of antigen-presenting (DC-like) cells in primary brain cell culture.
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| Details |
Background information
GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Mouse GM-CSF activity assay. The biological activity of Mouse GM-CSF was determined by proliferation assay using mouse FDC-P1 cells. The bioassay was calibrated with the non WHO reference material for mouse GM-CSF (NIBSC code 91/658) provided by the National Institute for Biological Standards and Control. |
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| Figure 2 |
| SDS-PAGE of Mouse GM-CSF, premium grade under reducing (R) and non-reducing (NR) conditions. |
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| Details |
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| References |
| 1. Ait-Oufella et al. (2010) J. Exp. Med. 207:1579-1587 |
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