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| Overview |
FGF-1 stands for fibroblast growth factor 1, also termed acidic fibroblast growth factor (aFGF). Human FGF-1 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human FGF-1 can be used for a variety of applications, including:
- Differentiation of embryonic and adult stem cells, e.g. into hepatocyte-like cells or pulmonary progenitors.
- In vitro proliferation of different cell types, including endothelial cells, cardiomyocytes, and skeletal muscle satellite cells.
- Differentiation or transdifferentiation of mesenchymal stromal cells.
- Investigation of FGF-1 mediated signaling pathways, e.g. regulation of apoE-HDL production in astrocytes.
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| Details |
Background information
FGF-1 belongs to the family of heparin-binding proteins. It acts as mitogen for a broad range of cells, including most cells of mesodermal and ectodermal origin. The adult brain is one of the most abundant sources of FGF-1. As a potent neurotrophic factor, FGF-1 affects survival and differentiation of neuronal cells both in vitro and in vivo. In addition, FGF-1 has angiogenic activity and promotes endothelial cell proliferation in vitro and blood vessel growth and vascular repair in vivo. The involvement in angiogenesis is also of critical importance in tumor growth and progression.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
| Alternative names |
| acidic FGF, aFGF, HBGF-1 |
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| Figure 1 |
| Human FGF-1 activity assay. The biological activity of Human FGF-1 is determined by proliferation assay using 3T3 cells in the presence of sodium heparin. |
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| Figure 2 |
| SDS-PAGE of Human FGF-1, premium grade under reducing (R) and non-reducing (NR) conditions. |
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