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| Overview |
SCF stands for stem cell factor, also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF). Human SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human SCF can be used for a variety of applications, including:
- Stimulation of proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures.
- In vitro expansion of CD34+ hematopoietic progenitor cells.
- Differentiation of ES-derived cells towards the hematopoietic lineage.
- Mast cell differentiation and maintenance.
- Mast cell chemotaxis assays.
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| Details |
Background information
SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
| Alternative names |
| c-kit ligand, steel factor, MGF |
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| Figure 1 |
| Human SCF activity assay. The biological activity of Human SCF is determined by proliferation assay using TF-1 cells. |
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| Figure 2 |
| SDS-PAGE of Human SCF, premium grade under reduced (R) and non-reduced (NR) conditions. |
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