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| Overview |
G-CSF stands for granulocyte colony stimulating factor. Human G-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human G-CSF can be used for a variety of applications, including:
- Growth promotion and differentiation of cell of the neutrophil lineage.
- Induction of colony formation of normal and leukemic bone marrow cells in soft agar cultures.
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| Details |
Background information
G-CSF is a hematopoietic growth factor that affects proliferation and differentiation especially of progenitors of the neutrophil and granulocyte lineages. It is produced mainly by monocytes and macrophages and a variety of other cells like astrocytes, fibroblasts and endothelial cells in response to specific stimulation, for instance by endotoxin, TNF-α and IFN-γ. Furthermore, G-CSF enhances the survival and influences the immunological functions of mature neutrophils. Thus, in addition to its properties as a hematopoietic growth factor G-CSF also acts as a mediator of host defense against infection and inflammatory response.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Human G-CSF activity assay. The biological activity of Human G-CSF is determined by proliferation assay using NFS-60 cells. |
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| Figure 2 |
| SDS-PAGE of Human G-CSF, premium grade under reduced (R) and non-reduced (NR) conditions. |
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