Human FGF-2 ( #130-093-839 #130-093-840 #130-093-841 #130-093-842 #130-093-843 #130-093-564 #130-093-837 #130-093-838 )

Human FGF-2

Overview

FGF-2 or FGF2 stands for fibroblast growth factor 2, also termed fibroblast growth factor basic (FGF-b) or basic FGF. Human FGF-2 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Human FGF-2 can be used as a cell culture supplement to optimize the culture conditions for a broad variety of cell types, such as mesenchymal stromal cells (MSCs), neural cells, and endothelial cells. Human FGF-2 also promotes long-term maintenance and propagation of undifferentiated embryonic and induced pluripotent stem cells.

Details

Background information
FGF-2 belongs to the family of heparin-binding growth factors. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Alternative names

basic FGF, HBGF-2

Figure 1

Human FGF-2 activity assay. The biological activity of Human FGF-2, premium grade was determined by proliferation assay using 3T3 cells.

Figure 2

In vitro generation of primary mouse neurospheres. A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1⁺ cells were cultivated for 7 days in MACS NeuroMedium containing MACS Supplement B27 PLUS, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).

<b><i>In vitro</i> generation of primary mouse neurospheres.</b> A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1<sup>+</sup> cells were cultivated for 7 days in MACS NeuroMedium containing MACS Supplement B27 PLUS, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).