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| Overview |
FGF-8b stands for fibroblast growth factor 8b. Human FGF-8b is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human FGF-8b can be used for a variety of applications, including:
- In vitro expansion of embryonic stem cell-derived neural progenitor cells.
- Survival of neural precursor cells and differentiation into astroglial cells.
- Stimulation of osteoblast proliferation.
- Proliferation of endothelial cells.
- Proliferation of myogenic cells.
- Tumor growth studies of prostate and breast cancer.
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| Details |
Background information
FGF-8, originally identified as androgen-induced growth factor, is a heparin-binding protein, which has mitogenic and transforming activity. Eight secreted FGF-8 isoforms (FGF-8a to h) have been identified in mice, whereas four have been described in humans (a, b, e, and f). FGF-8b appears to be the predominant form and has been shown to have the most important oncogenic transforming capacity. FGF-8 exerts a pivotal role in embryogenesis. It is expressed during gastrulation and influences brain, limb, heart and facial development in the mouse embryo. In addition, FGF-8 was shown to stimulate osteoblast proliferation. The amino acid sequence of human FGF8-b shares 100% identity with mouse FGF-8b.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by B27 supplements and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression. |
| 1: TH (tyrosine hydroxylase)+ cells |
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| 2: TuJ1 (neuron-specific β-III tubulin)+ cells |
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| 3: GAD67 (glutamate decarboxylase)+ cells |
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| Figure 2 |
| Human FGF-8b activity assay. The biological activity of Human FGF-8b is determined by proliferation assay in the presence of sodium heparin using 3T3 cells. |
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| Figure 3 |
| SDS-PAGE of Human FGF-8b, premium grade under reduced (R) and non-reduced (NR) conditions. |
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