Stemfactor™ BMP-4 (Human Recombinant) ( #130-095-549 )

Stemfactor™ BMP-4 (Human Recombinant)

Description

Stemfactor™ BMP-4 (Human Recombinant) was stringently tested for bioactivity, low endotoxin levels, high purity, and structural homogeneity, and is ideal for studying differentiation in human embryonic stem (ES) cells and maintaining pluripotency in mouse ES cell culture applications. Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGF-β protein superfamily. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair¹. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis^2,3. BMP-4 plays an important role in differentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays distinct role in mouse and human ES cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal⁴. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human ES cells. Stemfactor™ BMP-4 (Human Recombinant), is expressed and purified from human 293 cells as a glycosylated homodimer with an apparent molecular mass of 34-38 kDa.

Specifications and technical documents

Specification Sheets

03-0007 Specifications Sheet

Material Safety Data Sheets

Recombinant Protein Material Safety Data Sheet

Figure 1

Immunocytochemistry analysis of mouse ES cells cultured in the presence of BMP-4 and LIF.