CD146 ( #130-092-851 #130-092-853 #130-092-849 #130-092-852 #130-092-850 )

CD146

Overview

The CD146 antibodies have been designed to identify and enumerate human CD146⁺ cells by flow cytometry, fluorescence microscopy, or immunohistochemistry.

Clone 541-10B2 recognizes the human CD146 antigen, also known as MUC18, MCAM, Mel‑CAM, and S-Endo-1.

Details

Background information
CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains 5 extracellular immunoglobulin (Ig)-like domains.^1,2 CD146 possesses a limited tissue expression distribution, including endothelial cells, pericytes, smooth muscle cells, follicular dendritic cells, melanoma cells, and a sub-population of activated T lymphocytes.^1,3 CD146 is also expressed on marrow stromal cells (MSCs).⁴
CD146 functions as a Ca²⁺-independent cell adhesion molecule that is involved in heterophilic cell-cell interactions⁵, and may be involved in the extravasion and/or homing of activated T cells³. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.^6,7

Clone	Isotype
541-10B2	Mouse IgG1

Figure 1

Human umbilical vein endothelial cells (HUVECs) were passaged three times in culture and then stained with CD146 antibodies conjugated to FITC (A), PE (B), and APC (C), or CD146-Biotin and Anti-Biotin-PE (D), and analyzed by flow cytometry (black line). Fc receptor–mediated binding was prevented with the use of FcR Blocking Reagent, human. Unstained HUVECs were used as a negative control (gray line).

A: HUVECs stained with CD146-FITC.

B: HUVECs stained with CD146-PE.

C: HUVECs stained with CD146-APC.

D: HUVECs stained with CD146-Biotin and Anti-Biotin-PE.