MC CD34/CD133 Stem Cell Cocktail (human) ( #130-092-882 )

MC CD34/CD133 Stem Cell Cocktail (human)

Overview

MC Antibody Cocktails simplify the flow cytometric evaluation of cells separated by MACS® Technology. The MC CD34/CD133 Stem Cell Cocktail is designed for easy and rapid evaluation of cell separations using the CD133 MicroBead Kit, human. The cocktail is optimized for separation control using the MACSQuant® Analyzer, but can be used on other flow cytometers as well.

Details

Background information
The MC CD34/CD133 Stem Cell Cocktail consists of two components: the CD34/CD133 cocktail and a control cocktail. Whereas the CD34/CD133 cocktail includes CD133, CD34, and CD45 antibodies for optimal identification of hematopoietic stem and progenitor cells, the control cocktail additionally contains excess unconjugated CD133 antibody to block the staining of CD133⁺ cells. Thus, false positive events are reliably identified and excluded from the analysis. In addition, CD45-VioBlue is included in both cocktails as a trigger to restrict analysis to leukocytes. The CD45-VioBlue antibody recognizes a different epitope than the CD45-FITC antibody. VioBlue requires the use of a violet laser for flow cytometric detection.

Clone	Isotype
CD133/2-PE	Mouse IgG2b
CD34-APC	Mouse IgG2a
CD45-FITC	Mouse IgG1
CD45-VioBlue	Mouse IgG2a

Human early progenitor cells were enriched from PBMCs using the CD133 MicroBead Kit. Aliquots of cells before and after separation were stained with the MC CD34/CD133 Stem Cell Cocktail and analyzed by flow cytometry. CD133⁺ target cells are gated in region P4.

A: before separation

B: after separation