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| Overview |
The PE-conjugated monoclonal antibody CD117 (AC126) has been developed for
- the detection and quantification of CD117+ cells by flow cytometry or fluorescence microscopy,
- indirect labeling for the isolation of CD117+ cells in combination with Anti-PE MicroBeads. Since CD117(AC126)-PE does not interfere with stem cell factor (SCF)-binding, isolated cells remain responsive to SCF stimulation.
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| Details |
Background information
CD117 (also known as c-kit or SCF receptor) is a 145 kDa receptor tyrosine kinase involved in stem cell signaling, activation, and proliferation. The CD117 antigen is expressed on about 1–3% of PBMCs and cord blood cells and on up to 10% of bone marrow cells. CD117 is further expressed on basophils, myeloid dendritic cells, TCRα/β+ T cells, CD19+ B cells, and CD56+ NK cells1, as well as on mast cells, melanocytes, and acute myeloid leukemia (AML) blasts2. |
| Clone | Isotype |
| AC126 | Mouse IgG1 |
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| Figure 1 |
| Human cord blood mononuclear cells (CB-MNCs) were stained with CD117 (AC126)-PE and CD133/2 (293C3)-APC, and analyzed by flow cytometry. |
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| Favorites / Prices |
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| Details |
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| Products |
| CD117 (AC126)-PE, human |
- for 100 tests (1)
Download datasheet
130-091-735
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Guth et al. (1995) (Abstract) 9th International Congress of Immunology. |
| 2. Bühring et al. (1991) Leukemia 5: 854–860. |
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