CD133/1 (W6B3C1) - for Western blot analysis

Proven and tested: tool of choice in more than 500 publications
Exclusive: developed by Miltenyi Biotec, available from Miltenyi Biotec only
Unique: CD133 antibodies for analysis of primitive stem cells

Overview

The antibody CD133 (W6B3C1) has been optimized for the evaluation of CD133 expression by Western blot analysis.

Details

Background information
CD133 is found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. in human fetal liver, bone marrow, cord blood, and peripheral blood. CD133 has also been found to be expressed on circulating endothelial progenitor cells and other tissue cells such as brain^1,2, prostatic tissue³, muscle⁴, or stroma of the cornea⁵, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells.

Clone	Isotype
W6B3C1	mouse lgG1

Further information

Stem Cell Research Brochure
[PDF; 3,8 MB]
Neuroscience research brochure
[PDF; 1,7 MB]
Cancer Research Brochure
[PDF; 4,3 MB]

Figure 1

Western blot analysis of 2×10⁵ PBMCs (lane 1) spiked with 10% (lane 2), 5% (lane 3), 1% (lane 4), 0.5% (lane 5), and 0.2% (lane 6) WERI-Rb-1 cells, a CD133-expressing cell line. M: marker.

Western blot analysis of 2×10<sup>5</sup> PBMCs (lane 1) spiked with 10% (lane 2), 5% (lane 3), 1% (lane 4), 0.5% (lane 5), and 0.2% (lane 6) WERI-Rb-1 cells, a CD133-expressing cell line. M: marker.

Figure 2

Protein extracts of human hippocampus (Lane 2), human fetal brain (Lane 3) and Weri-Rb-1 cells (Lane 5) were blotted onto a PVDF membrane and probed with CD133/1 (W6B3C1) pure antibody. Lane 4 is empty. Goat anti-mouse IgG-HRP was applied at a dilution of 1:10,000 before bands were developed using ECL Plus™ chemiluminescent detection reagent. The molecular weight marker (Lane 1) indicates CD133 reactivity at approximately 120 kDa in Lanes 3 and 5.