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| Overview |
| The CD45 antibody can be used to identify and enumerate human CD45+ cells by flow cytometry or fluorescence microscopy. CD45, also known as leukocyte common antigen (LCA) is present on all human cells of hematopoietic origin, except erythroid cells, platelets, and their precursor cells. |
| Details |
Background information
The CD45 antibody recognizes the human CD45 antigen, a tyrosine phosphatase also known as the leukocyte common antigen (LCA). The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms. |
| Clone | Isotype |
| 5B1 | Mouse IgG2a |
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| Figure 1 |
| Lysed human whole blood was stained with CD45 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (E). Human peripheral blood mononuclear cells were stained with CD45 antibodies conjugated to VioGreen (F), PE-Vio770 (G), or APC-Vio770 (H). Cells were analyzed using the MACSQuant® Analyzer. |
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