|
|
 |
|
| Overview |
| The CD324 (E-Caherin) antibody can be used for the flow cytometric identification and enumeration of CD324 (E-Cadherin)+ cells, e.g. human ES and iPS cells, carcinoma cells, human cells of epithelial origin. |
| Details |
Background information
CD324, also known as E-Cadherin or cadherin-1, is a calcium dependent cell adhesion protein found in adherence junctions of epithelial cells, e.g., in colon, uterus, liver, keratinocytes etc. E-Cadherin also serves as a ligand for integrin alpha-E/beta-71. The epitope is expressed by a variety of carcinoma-derived cell lines such as MCF-7, HT-29, T-47D, NTERA-2, and 2102Ep. Loss of E-Cadherin is thought to enable metastasis by disrupting intercellular contacts—an early step in metastatic dissemination2. Furthermore, E-Cadherin is a marker of undifferentiated embryonic stem (ES) cells and induced pluripotent stem (iPS) cells3,4. For these cells, E-Cadherin plays a major role in conveying cell survival signals5. |
| Clone | Isotype |
| 67A4 | mouse IgG1 |
| |
|
| Human induced pluripotent stem (iPS) cells were stained with CD324 (E-Cadherin) antibodies conjugated to PE (A) or APC (B) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Right line represents CD324 (E-Cadherin) staining, left line represents isotype control. |
 |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| CD324-PE, human |
- for 100 tests (1)
Download datasheet
130-095-413
|
| CD324-APC, human |
- for 100 tests (1)
Download datasheet
130-095-412
|
| (1) One test corresponds to fluorescent labeling of up to 106 cells in a total volume of 100 μL. |
|
| References |
| 1. Higgins, J. M. et al. (1998). J. Cell Biol. 140: 197–210. |
| 2. Onder, T. T. et al. (2008 Cancer Res. 68: 3645–3654. |
| 3. Eastham, A. M. et al. (2007) Cancer Res. 67: 11254–11262. |
| 4. Li, Z. et al. (2010) J. Phys. Chem. B. 114: 2894–2900. |
| 5. Xu, Y. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107: 5129–8134. |
|
| MACS Stem Cell newsletter |
|
Stay current with important developments in stem cell research by subscribing to
Stem Cell Updates.
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
