Stemgent® DyLight™ 488 Mouse anti-Mouse/Human SSEA-1 Antibody ( #130-095-671 )

Stemgent® DyLight™ 488 Mouse anti-Mouse/Human SSEA-1 Antibody

Description

Stemgent® DyLight™ 488 Mouse anti-Mouse/Human SSEA-1 Antibody was screened on the mouse embryonic stem cell line R1 using immunofluorescent staining and flow cytometry (FC), and selected as the best conjugated anti-SSEA-1 antibody available for researchers needing to demonstrate mouse ES/iPS cells pluripotency. The MC-480 antibody reacts with mouse and human stage-specific embryonic antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration and differentiation.

Disclaimer

This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel.

Clone	Isotype
MC-480	Mouse IgMκ

Specifications and technical documents

Specification Sheets

09-0070 Specifications Sheet

Material Safety Data Sheets

General Antibody MSDS

Protocols

Figure 1

The mouse embryonic stem cell line R1 was stained with Stemgent® Dylight™ 488-conjugated anti-SSEA-1 (Cat. No. 09-0070, red filled histogram) or Stemgent® DyLight™ 488 Goat anti-Mouse IgG + IgM (Cat. No. 09-00033), black open histogram) at the same.

Figure 2

Mouse embryonic stem cell line R1 was fixed and expression of SSEA-1 was examined by immunocytochemistry with Stemgent® Dylight™ 488- conguagated anti-SSEA-1 (Cat. No. 09-0070, green color). DAPI staining was performed to visualize nuclei (blue color).