|
|
 |
| Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody |
|
| Description |
| The Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody was screened on live, unfixed human embryonic stem (ES) cells using immunocytochemistry (ICC). This antibody was selected as the best conjugated TRA-1-60 antibody available for visualization of ES/iPS (induced pluripotent stem) cells, utilizing direct fluorescent staining and real-time imaging without fixation or killing the cells. This antibody format is useful for direct visualization of the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi, mouse virus and mycoplasma as tested. The live ES cell morphology, proliferation and expression of pluripotency markers did not change after staining with this antibody. Clone TRA-1-60 reacts with a pluripotent stem cell-specific antigen expressed on undifferentiated human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. |
| Disclaimer |
| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
| Clone | Isotype |
| TRA-1-60 | Mouse IgMκ |
| Specifications and technical documents |
| Specification SheetsMaterial Safety Data SheetsProtocols
|
| |
|
| Figure 1 |
| ICC Analysis of StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody on Human H1 ES Cells. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| Stemgent StainAlive DyLight 488 Mouse anti-Human TRA-1-60 Antibody |
- 50 µg
- Stemgent Catalog # 09-0068
130-095-669
|
|
| References |
| 1. Takahashi et al. (2007) Cell 131: 861–872. |
| 2. Yu et al. (2007) Science 318: 1917–1920. |
| 3. Schopperle and DeWolf (2007) Stem Cells 25: 723–730. |
| 4. Chin et al. (2007) J Biotechnol. 130: 320–328. |
| 5. Badcock et al. (1999) Cancer Res. 59: 4715–4719. |
| 6. Andrews et al. (1984) Hybridoma 3: 347–361. |
| 7. Lowry et al. (2008) Proc Natl Acad Sci U S A 105: 2883–2888. |
|
| MACS Stem Cell newsletter |
|
Stay current with important developments in stem cell research by subscribing to
Stem Cell Updates.
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
