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| Overview |
| The Anti-A2B5 antibody binds to glial progenitors from embryonic to adult human, murine, and rat tissue1. It is optimized for the identification of A2B5+ glial precursor cells by flow cytometry or fluorescence microscopy2–6. |
| Details |
Background information
The clone 105HB29 recognizes the c-series ganglioside–specific antigen A2B5.
A2B5 is predominantly expressed in embryonic and neonatal neural tissue. In the adult mammalian brain, A2B5 expression is restricted mainly to areas which retain neurogenic potential such as the subventricular zone (SVZ). Thus, A2B5 is considered as a marker for immature glial-committed precursors which are permanently generated in the SVZ. Glial precursor cells are defined as cells that give rise to glial cell types, such as astrocytes and oligodendrocytes.
Ganglioside GT3 and its O-acetylated derivative are the principal A2B5-reactive gangliosides7, and both antigens are down-regulated as the cells differentiate into mature oligodendrocytes. The Anti-A2B5 antibody can be used to monitor the maturation of oligodendrocyte progenitors. |
| Clone | Isotype |
| 105HB29 | Mouse IgM |
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| Figure 1 |
| Mouse brain tissue postnatal day 1 was dissociated using the Neural Tissue Dissociation Kit (T). Brain cells were stained with Anti-A2B5 antibodies conjugated to PE (A), APC (B), or Biotin (C) and analyzed by flow cytometry. Cells labeled with Anti-A2B5-Biotin (C) were stained with Anti-Biotin-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| B |
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| Details |
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| Products |
| Anti-A2B5-PE, human, mouse, rat |
- for 100 tests (1)
Download datasheet
130-093-581
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| Anti-A2B5-APC, human, mouse, rat |
- for 100 tests (1)
Download datasheet
130-093-582
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| Anti-A2B5-Biotin, human, mouse, rat |
- for 100 tests (2)
Download datasheet
130-093-393
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| Anti-A2B5 pure, human, mouse, rat |
Download datasheet
130-093-394
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| (1) One test corresponds to fluorescent labeling of up to 106 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 106 cells in a total volume of 100 μL. |
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| References |
| 1. Dietrich et al. (2002) Glia 40: 65–77. |
| 2. Larsen, P.H. et al. (2003) J. Neurosci. 23: 11127–11135. |
| 3. Ruffini et al. (2004) Am. J. Pathol. 165: 2167–2175. |
| 4. Windrem et al. (2004) Nat. Med. 10: 93–97. Erratum in: Nat. Med. (2004) 10: 209. |
| 5. Larsen, P.H. and Yong, V.W. (2004) J. Neurosci. 24: 7597–7603. |
| 6. Larsen et al. (2006) J. Neurosci. 26: 2207–2214. |
| 7. Dubois et al. (1990) J. Biol. Chem. 265: 2797–2803. |
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