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| Overview |
| The antibody is suitable for the identification and enumeration of mouse CD140b+ cells by flow cytometry. |
| Details |
Background information
Clone APB5 reacts with the transmembrane glycoprotein CD140b, the β chain of the platelet-derived growth factor receptor (PDGFR). The two isoforms of the receptor can form homodimers (α/α or β/β) or α/β heterodimers. Receptor dimerization and transphosphorylation at tyrosine residues activates the intracellular kinase activity. CD140b has been reported to be broadly expressed in embryonic tissue, mostly in cells of mesodermal origin in adult tissue. Expression of CD140a is associated with various malignancies. |
| Clone | Isotype |
| APB5 | Rat IgG2a |
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| Figure 1 |
| Mouse NIH-3T3 cells mixed with mouse 1881 cells were stained with CD140b antibodies conjugated to PE (A) or APC (B) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD104b-Biotin (C) were stained with Anti-Biotin-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| C |
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| Favorites / Prices |
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| Details |
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| Products |
| CD140b-PE, mouse |
- for 100 tests (1)
Download datasheet
130-096-267
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| CD140b-APC, mouse |
- for 100 tests (1)
Download datasheet
130-096-270
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| CD140b-Biotin, mouse |
- for 100 tests (2)
Download datasheet
130-096-272
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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