Anti-Feeder ( #130-096-094 #130-096-099 #130-096-095 )

Anti-Feeder

Overview

The Anti-Feeder Antibodies have been specifically developed for the detection of mouse feeder cells in feeder-dependent co-cultures. They are optimally suited for:

Gating-out of feeder cells during flow cytometric analysis of stem cell cultures.
Identification and enumeration of cultivated mouse feeder cells by flow cytometry or fluorescence microscopy.
Evaluation of MACS® Separations by flow cytometry or fluorescence microscopy. Mouse feeder cells can be depleted by using Feeder Removal MicroBeads, mouse

Details

Background information
The most prevalent cultivation method for maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is co-cultivation on primary mouse embryonic fibroblast (mEF) feeder cell layers. Feeder cell layers of NIH3T3 fibroblasts or stromal derived OP9 cells are also frequently used in co-culture systems: NIH3T3 fibroblasts support primary keratinocyte cultures, OP9 cells are used for hematopoietic induction of ES cells.

Clone	Isotype
mEF-SK4	Rat IgG1

Further information

Feeder Removal Poster ISSCR 2010
[PDF; 548,6 KB]

Co-cultures of mouse embryonic stem (mES) cells and mouse embryonic fibroblast (mEF) cells were stained with Anti-Feeder-PE (A), -APC (B), or -Biotin (C) and Anti-Biotin-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. mEF cells could be clearly discriminated from unstained mES cells based on the Anti-Feeder antibody staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.