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| Stemgent® Alkaline Phosphatase Staining Kit II |
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| Description |
The Stemgent® Alkaline Phosphatase (AP) Staining Kit II detects alkaline phosphatase activity, a phenotypic marker of pluripotent stem cells.
Undifferentiated embryonic stem (ES), induced pluripotent stem (iPS) embryonic germ (EG) and embryonal carcinoma (EC) cells exhibit elevated expression of alkaline phosphatase (AP). The state of differentiation can be monitored by detection of AP along with stem cell markers such as transcription factors Oct-3/4, Nanog, Sox-2 and surface markers SSEA-1, -3, -4, TRA-1-60 and TRA-1-81. Although stem cells from different sources or species display different cell surface markers, AP is a universal marker for all types of pluripotent stem cells.
Alkaline phosphatase (AP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. There are various methods of detecting AP such as those based on enzymatic reaction followed by colorimetric or fast red violet dye, and others based on fluorescent detection and immunostaining. The Stemgent® Alkaline Phosphatase Staining Kit II is a histochemical assay for use on cells grown in tissue culture wells or dishes. It uses a solution containing napthol AS-BI phosphate as substrate and fast red violet dye as the colorimetric read-out for the enzymatic activity. The AP-positive, undifferentiated cells stain red. For added convenience, the activated substrate can be used up to 30 minutes after mixing. The stain develops quickly, does not readily fade, and can be analyzed from 15 to 30 minutes after application to the sample. |
| Disclaimer |
| This Stemgent® Product is exclusively distributed by Miltenyi Biotec outside the USA and Israel. |
| Specifications and technical documents |
| Specification SheetsMaterial Safety Data SheetsProtocols
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| Figure 1 |
| AP staining of mouse R1 ES cells on MEF feeder layer. |
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| Figure 2 |
| AP staining of human H1 ES cells on MEF feeder layer. |
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| Details |
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| Products |
| Stemgent Alkaline Phosphatase Staining Kit II |
- 50 assays
- Stemgent Catalog # 00-0055
130-095-616
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| References |
| 1. Pease et al. (1990) Dev. Biol. 141: 344–352. |
| 2. Smith et al. (1992) Dev Biol 151: 339–351. |
| 3. Takahashi et al. (2007) Cell 131: 861–872. |
| 4. Yu et al. (2007) Science 318: 1917–1920. |
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