Anti-PSA-NCAM MicroBeads ( #130-092-966 )

Anti-PSA-NCAM MicroBeads

Overview

Anti-PSA-NCAM MicroBeads have been developed for the positive selection or depletion of PSA-NCAM⁺ cells from tissue or cell culture.

Details

Background information
Anti-PSA-NCAM MicroBeads recognize polysialic acid (PSA), which is linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56).¹ PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue². In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ)³ and the dentate gyrus of the hippocampus.⁴ PSA-NCAM is a marker for immature neuronal-committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons.^3,5 PSA-NCAM positive neuronal precursors have been isolated from rat forebrain and from mouse SVZ tissue after depletion of A2B5⁺glial progenitor cells.^6-10

Columns

For positive selection: MS, LS, or autoMACS Columns.
For depletion: LD or autoMACS Columns.

Further information

Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells
[PDF; 310,8 KB]
Purification of neuronal precursors – customer report
[PDF; 228,2 KB]

Figure 1

Separation of a single-cell suspension derived from P1 mouse whole-brain tissue using the Neural Tissue Dissociation Kit (T), Anti-PSA-NCAM MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with rat anti-mouse IgM-APC and analyzed by flow cytometry.

A: Neural cells before separation

B: PSA-NCAM^- cells

C: PSA-NCAM⁺ cells