Anti-TRA-1-60 MicroBead Kit ( #130-095-816 )

Anti-TRA-1-60 MicroBead Kit

Overview

The Anti-TRA-1-60 MicroBead Kit has been developed for the separation of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.

The Anti-TRA-1-60 MicroBead Kit enables the:

positive selection of undifferentiated TRA-1-60⁺ pluripotent stem cells, for example, human ES and iPS cells,
isolation of undifferentiated cells from differentiated cell populations,
efficient enrichment of iPS cells during or after reprogramming¹.

Details

Background information
The TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.^2,3 The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34- related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer.

Columns

LS or autoMACS Columns

Further information

Sequential enrichment of TRA-1-60+ PSC and CD271 (p75)+ NCSC for peripheral neuron differentiation_Knoebel et al_2011
[PDF; 237,4 KB]
Stem Cell Research Brochure
[PDF; 3,8 MB]
Stem Cell Product List Sept 2011
[PDF; 709,8 KB]

Figure 1

Pluripotent TRA-1-60⁺ iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using the Anti-TRA-1-60 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with Stemgent® Anti-TRA-1-60-PE Antibody and CD326-APC, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

Pluripotent TRA-1-60<sup>+</sup> iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using the Anti-TRA-1-60 MicroBead Kit, an <a href="/en/PG_115_163_LS_Columns.aspx">LS Column</a>, and a <a href="/en/PG_124_182_MidiMACS_Separator.aspx">MidiMACS™ Separator</a>. Cells were fluorescently stained with <a href="/en/PG_884_1273_Stemgent_Phycoerythrin_PE_anti_Human_TRA_1_60_Antibody.aspx">Stemgent® Anti-TRA-1-60-PE Antibody</a> and <a href="/en/PG_595_229_CD326_EpCAM_.aspx">CD326-APC</a>, and analyzed by flow cytometry using the <a href="http://www.macsquant.com">MACSQuant® Analyzer</a>. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Enriched TRA-1-60⁺ pluripotent stem cells