Anti-SSEA-1 (CD15) MicroBeads ( #130-094-530 )

Anti-SSEA-1 (CD15) MicroBeads

Overview

Anti SSEA-1 (CD15) MicroBeads are the first product to allow magnetic cell separation based on the expression of SSEA-1. In less than an hour, it is possible to

separate pluripotent mouse ES or iPS cells from heterogeneous cultures, e.g. to eliminate feeder cells,
enrich reprogrammed mouse iPS cells,
deplete unwanted pluripotent cells from ES or iPS cell-derived differentiation cultures,
deplete human ES or iPS cells that have undergone spontaneous differentiation.

Details

Background information
In the murine system, SSEA-1 is one of the key embryonic stem (ES) cell markers associated with the state of pluripotency. It is known to be expressed on undifferentiated murine ES and induced pluripotent stem (iPS) cells.^1-4 In contrast, SSEA-1 is not expressed on undifferentiated human ES cells but becomes upregulated during spontaneous differentiation.⁵

Columns

For positive selection: MS, LS, XS, or autoMACS Columns. For depletion: LD, CS, D, or autoMACS Columns.

Figure 1

Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K^kwere taken either before or after MACS® Separation and stained with anti-H-2K^k-PE before analysis by flow cytometry. Expression of ectopic H-2K^k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation.

Before separation

Negative fraction

$Negative fraction$

Isolated mouse ES cells