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MACS® Media MACS® Media
MACS® Cytokines MACS® Cytokines
MACS® Cell Stimulation Reagents MACS® Cell Stimulation Reagents
  T Cell Activation/Expansion Kit, human
  Treg Expansion Kit, human
  Treg Suppression Inspector, human
  T Cell Activation/Expansion Kit, non-human primate
  T Cell Activation/Expansion Kit, mouse
  Treg Expansion Kit, mouse
  NK Cell Activation/Expansion Kit, human
  MSC Suppression Inspector Kit, human
  Anti-Biotin MACSiBead™ Particles, cell culture grade
  Functional grade antibodies
  CytoStim
  Peptide pools
  CMV pp65 – Recombinant Protein
  CMV IE-1 – Recombinant Protein
Antigen targeting Antigen targeting
Culture bags Culture bags
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Treg Expansion Kit, mouse
Description
The Treg Expansion Kit was developed for the efficient expansion of murine Treg cells.
Details
Background information
This kit sustains FoxP3 expression in expanded Treg cells that were previously isolated with the CD4+CD25+ Regulatory T Cell Isolation Kit, mouse. The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 3:1 and recombinant interleukin 2 (rIL-2) at a concentration of 2,000 U/mL.

Downstream applications
Expanded Treg cells can be used for any downstream application, such as adoptive transfer experiments, cytokine analysis, gene expression studies, and suppression assays.
 
Figure 1
Mouse Treg cells were isolated using the CD4+CD25+ Regulatory T Cell Isolation Kit and expanded for 13 days with the Treg Expansion Kit. Treg cells before (A, B) and after expansion (C, D) were fluorescently stained with the Treg Detection Kit (CD4/CD25/FoxP3) (APC).
A: Treg cells before expansion
B
C: Expanded Treg cells
D
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Treg Expansion Kit, mouse
- 2 mL (1)
Download datasheet
130-095-925
Qty.:
 

(1) 2 mL correspond to 1.2×108 MACSiBead™ Particles, cell culture grade, pre-loaded with CD3 and CD28 antibodies.
Related products
CD4+CD25+ Regulatory T Cell Isolation Kit
Mouse IL-2
Anti-FoxP3 antibodies
gentleMACS Dissociator
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