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| Treg Expansion Kit, human |
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| Description |
| The Treg Expansion Kit was developed for the efficient expansion of human Treg cells. |
| Details |
Background information
Using this kit FoxP3 expression by Treg cells is sustained after isolation with the CD4+CD25+CD127dim/– or CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kits from human blood or PBMCs.1 The kit is also suitable for usage with rapamycin.2
The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 4:1 and recombinant interleukin 2 (rIL-2) with a concentration of 500 U/mL.
Downstream applications
Expansion of human Treg cells after isolation with the CD4+CD25+CD127dim/– or CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kits from human blood or PBMCs. Expanded Treg cells can be used for any downstream application such as cytokine analysis, gene expression, and suppression assays. |
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| Figure 1 |
| Treg cells were isolated from human PBMCs by using the CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit II and expanded with the Treg Expansion Kit for 14 days. Treg cells before (A,B) and after expansion in the absence (C,D) or presence (E,F) of rapamycin were fluorescently stained and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression. |
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| A: Isolated Treg cells |
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| B |
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C: Expanded Treg cells (without rapamycin)
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| D |
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E: Expanded Treg cells
(with rapamycin) |
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| F |
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| Figure 2 |
| Expansion rates of Treg cells were calculated from the numbers of FoxP3-expressing cells before and after 14 days of expansion in the absence or presence of rapamycin. |
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| Details |
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| Products |
| Treg Expansion Kit, human |
- 2 mL (1)
Download datasheet
130-095-345
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- 2×2 mL (1)
Download datasheet
130-095-353
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| (1) 2 mL correspond to 4×107 MACSiBead™ Particles, cell culture grade, pre-loaded with CD3 and CD28 antibodies |
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| References |
| 1. Hoffmann, P. et al. (2006) Blood 108: 4260–4267. |
| 2. Battaglia, M. et al. (2005) Blood 105: 4743–4748. |
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