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| TCRγ/δ+ T Cell Isolation Kit |
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| Description |
| The TCRγ/δ+ T Cell Isolation Kit has been developed for the isolation of untouched mouse TCRγ/δ+ T cells from single-cell suspensions of spleen, lymph nodes, or thymus. |
| Details |
Background information
T cells bearing γ/δ TCRs are a phenotypically and functionally conserved lineage of T cells which often constitutes the major T cell population in epithelial-rich tissues, e.g. intestine, skin, lung, and reproductive tract. In blood and lymphoid tissues they are found at low frequency. TCRγ/δ+ T cells have been shown to be involved in immune surveillance against tumors, wound healing, as well as pro- and anti-inflammatory responses against pathogens.1
The TCRγ/δ+ T Cell Isolation Kit contains CD11b and CD45R (B220) MicroBeads for depletion of CD11b+ and CD45R (B220)+ cells, as well as Anti-TCRγ/δ-Biotin and Anti-Biotin MicroBeads for positive selection of TCRγ/δ+ T cells.
Downstream applications
TCRγ/δ+ T cells isolated by using the TCRγ/δ+ T Cell Isolation Kit are suitable for phenotypic and functional analysis, adoptive transfer, and coculture experiments. |
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| Figure 1 |
| TCRγ/δ+ T cells were isolated from single cell suspensions of mouse spleen (A-C), lymph node (D), and thymus (E) by using the TCRγ/δ+ T Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. |
| A: Mouse spleen cells before separation |
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| B: Pre-enriched splenic TCRγ/δ+ T cells after depletion of CD11b+ and CD45R (B220)+ cells |
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| C: TCRγ/δ+ T cells isolated from spleen |
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| D: TCRγ/δ+ T cells isolated from peripheral lymph nodes |
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| E: TCRγ/δ+ T cells isolated from thymus |
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| Details |
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| Products |
| TCRγ/δ+ T Cell Isolation Kit, mouse |
- for 2×109 total cells
Download datasheet
130-092-125
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| References |
| 1. Carding et al. (2002) Nat. Rev. Immunol. 2: 336–345. |
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