|
|
 |
 - Unrivaled: excellent purity and unmatched recovery
- Time-saving: cells can be immediately used for downstream applications
- Reliable: proven in a plethora of publications
|
| Description |
| Mouse CD8a (Ly-2) MicroBeads have been developed for positive selection or depletion of mouse CD8a+ T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood. |
| Details |
Background information
The CD8a antigen is expressed on most thymocytes, almost all cytotoxic T cells, and on subpopulations of dendritic cells. CD8a functions as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on cytotoxic CD8a+ T cells.
Downstream applications
Purified CD8a+ cells have been used for in vitro and in vivo studies on protective immune responses against parasites1,2 or allergens3 and for adoptive transfer into immunodeficient4,5 and virus-infected mice.6 Highly pure CD8+ T cells were also isolated from the CNS of MHV-infected mice for evaluation of their chemokine expression pattern.7 Furthermore, CD8a MicroBeads have been used for the isolation of CD8+CD4+ (DP) thymocytes and the effect of PD-1 expression on their maturation was studied.8
|
| |
| Figure 1 |
| Positive selection of CD8a+ T cells from a mouse spleen cell suspension using CD8a (Ly-2) MicroBeads, an MS Column, and a MiniMACS™ Separator. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| CD8a (Ly-2) MicroBeads, mouse |
- for 2×109 total cells
Download datasheet
130-049-401
|
| CD8a (Ly-2) MicroBeads, mouse – lyophilized |
- for 2x109 total cells
Download datasheet
130-097-058
|
|
| References |
| 1. Schlüter et al. (1996) Am. J. Pathol. 150: 1021–1035. |
| 2. Zerrahn et al. (1996) J. Immunol. 156: 3919–3924. |
| 3. Hamelmann et al. (1996) J. Exp. Med. 183: 1719–1729. |
| 4. Harrison et al. (1996) J. Exp. Med. 184: 2167–2174. |
| 5. Hoerauf et al. (1996) Internat. Immunol. 8: 1569–1575. |
| 6. Johansen et al. (2004) Eur. J. Immunol. 34: 91–97. |
| 7. Liu et al. (2001) J. Immunol. 167: 4091–4097. |
| 8. Keir et al. (2005) J. Immunol. 175: 7372–7379. |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
