|
|
 |
| CD8a+ T Cell Isolation Kit II |
|
| Description |
| The CD8a+ T Cell Isolation Kit II was developed for the untouched isolation of CD8a+ T cells from single-cell suspensions from mouse spleen or lymph node. |
| Details |
Background information
The kit is based on a cocktail of biotin-conjugated antibodies against CD4, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, and Ter-119. The isolation of T cells is achieved by depletion of magnetically labeled cells.
Downstream applications
Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.
The CD8a+ T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments1,2 , T cell proliferation assays2,3 , Coculture with dendritic cells2 and Cytokine analysis experiments. |
| |
| Figure 1 |
| A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. T cells were isolated from this single-cell suspension using the CD8a+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD8a T Cell Cocktail, mouse and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| A: Before separation |
 |
| B: Isolated CD8a+ T cells |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| CD8a+ T Cell Isolation Kit II, mouse |
- for 109 total cells
Download datasheet
130-095-236
|
|
| References |
| 1. Hamilton-Williams et al. (2009) J. Immunol. 183: 1533–1541. |
| 2. Simma et al. (2009) Cancer Res. 69: 203–211. |
| 3. Grebe et al. (2009) Proc. Natl. Acad. Sci. USA 106: 5300–5305. |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
