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 - Unrivaled: excellent purity and unmatched recovery
- Time-saving: cells can be immediately used for downstream applications
- Reliable: proven in a plethora of publications
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| Description |
| Mouse CD4 (L3T4) MicroBeads were developed for positive selection or depletion of mouse T helper cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood. |
| Details |
Background information
The CD4 antigen is expressed on most thymocytes and mature T helper cells, and at lower levels on a subpopulation of dendritic cells. CD4 acts as an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4+ T helper cells. The CD4 antigen is expressed on approximately 90% of thymocytes, 25% of splenocytes, and 55% of lymph node cells.
Downstream applications
CD4+ T cells isolated with CD4 MicroBeads have been used for functional and phenotypical analysis of T cells in vitro and in vivo. The experiments comprised studies on T helper cell differentiation towards TH1 or TH2 cells,1 or studies on the regulatory role of cytokines in autoimmune disease2 and infection.3 Purified T helper cells were also used in a large panel of adoptive transfer experiments, e.g. for transfer into immunodeficient2,4,5, knock-out1, parasite infected6, and tumor-bearing mice7 or to passively transfer a disease with auto-antigen-stimulated CD4+ T cells in an EAE mouse model.8 Isolated CD4+ T cells have also been used to study the role of costimulatory molecules, such as OX40-OX40 Ligand interactions.9 Furthermore, CD4+ T cells were isolated from CNS suspensions of MHV infected mice for evaluation of their chemokine expression pattern.10 |
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| Figure 1 |
| Positive selection of CD4+ T cells from a mouse spleen cell suspension using CD4 (L3T4) MicroBeads, an LS Column, and a MidiMACS™ (A) or autoMACS® Separator, program 'Posseld' (B). |
| A: CD4+ T cells isolated using an LS Column and a MidiMACS™ Separator |
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| B: CD4+ T cells isolated with the autoMACS® Separator |
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| Details |
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| Products |
| CD4 (L3T4) MicroBeads, mouse |
- for 2×109 total cells
Download datasheet
130-049-201
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| References |
| 1. Schmitz et al. (1994) J. Exp. Med. 179: 1349. |
| 2. Trembleau et al. (1995) J. Exp. Med. 181: 817–821. |
| 3. Schlüter et al. (1996) Am. J. Pathol. 150: 1021–1035. |
| 4. Hoerauf et al. (1996) Int. Immunol. 8: 1569–1575. |
| 5. Johansen et al. (2004) Eur. J. Immunol. 34: 91–97. |
| 6. Khan and Kasper (1996) J. Immunol. 157: 2103–2108. |
| 7. Narvaiza et al. (2000) J. Immunol. 164: 3112–3122. |
| 8. Schmidt et al. (2005) J. Immunol. 175: 2286–2292. |
| 9. Soroosh et al. (2006) J. Immunol. 176: 5975–5987. |
| 10. Liu et al. (2001) J. Immunol. 167: 40-91–4097. |
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