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 - Unrivaled: excellent purity and unmatched recovery
- Time-saving: cells can be immediately used for downstream applications
- Reliable: proven in a plethora of publications
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| Description |
| The CD4+ T Cell Isolation Kit II was developed for the untouched isolation of CD4+ T cells from single-cell suspensions from mouse spleen or lymph nodes. |
| Details |
Background information
The kit is based on a cocktail of biotin-conjugated antibodies against CD8a, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, and Ter-119. The isolation of T cells is achieved by depletion of magnetically labeled cells.
Downstream applications
Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.
The CD4+ T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments1,2 , T cell proliferation assays1,2 , Coculture with dendritic cells3 and Cytokine analysis3 experiments. |
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| Figure 1 |
| A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. CD4+ T cells were isolated from this single-cell suspension using the CD4+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD4 T Cell Cocktail and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| A: Before separation |
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| B: Isolated CD4+ T cells |
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| Details |
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| Products |
| CD4+ T Cell Isolation Kit II, mouse |
- for 109 total cells
Download datasheet
130-095-248
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| References |
| 1. Miao et al. (2009) Blood 114: 4034–4044. |
| 2. Sharma et al. (2009) J. Immunol. 183: 1065–1073. |
| 3. Zhou et al. (2009) J. Virol. 83: 1625–1634. |
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