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| Description |
| CD90.2 MicroBeads were developed for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood. |
| Details |
Background information
The mouse CD90.2 alloantigen, also known as Thy1.2, is a pan T cell marker for the most common inbred mouse strains. These strains include BALB/c, DBA, CBA/J, C3H, C57BL/6, NZB/-, S3L and others. CD90.2 is expressed on thymocytes, peripheral T cells, on some intraepithelial T cells, and at lower levels on early hematopoietic stem cells in bone marrow.
The mouse CD90.2 antibody does not cross-react with CD90.1 (Thy1.1).
Downstream applications
T cells isolated with CD90.2 MicroBeads were used to analyze cytokine expression in a mouse model of lung fibrosis1 or after adoptive transfer of T cells from wild type and knock-out mice into immunodeficient mice.2 Furthermore, CD90.2 MicroBeads were used for the isolation of tumor-infiltrating T cells to evaluate their immunotherapeutic potential3 or functional properties.4 |
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| Figure 1 |
| Isolation of CD90.2 (Thy1.2)+ T cells from a mouse spleen cell suspension using CD90.2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were stained with CD90.2-FITC. |
| A: Before separation |
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| B: CD90.2- cells |
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| C: Isolated CD90.2+ cells |
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| Details |
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| References |
| 1. Huaux et al. (2003) J. Immunol. 170: 2083–2092. |
| 2. Roggia et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98: 13960–13965. |
| 3. Prévost-Blondel et al. (1998) J. Immunol. 161: 2187–2194. |
| 4. Radoja et al. (2000) J. Immunol. 164: 2619–2623. |
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