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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  Stem and progenitor cells
  Neural cells
  T cells
 
 
 
 
 
  NK cells
  B cells
  Monocytes and macrophages
  Myeloid derived suppressor cells
  Dendritic cells
  Antigen-presenting cells
  Granulocytes
  Leukocytes
  Endothelial cells
  Erythroid cells
  Cytokine-producing cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD5 (Ly-1) MicroBeads
Description
Mouse CD5 (Ly-1) MicroBeads are used for positive selection or depletion of T cells from single-cell suspensions of lymphoid or non-lymphoid tissue and peripheral blood.
CD5 is a T cell differentiation antigen expressed at high levels on most thymocytes, all mature T cells, and on a small subpopulation of B cells (CD5+ B-1a cells).
Applications
T cells isolated with CD5 MicroBeads were used to analyze cytokine secretion kinetics during T cell differentiation.1 For the isolation of highly pure T cells, B-1a cells can be depleted in advance by using CD45R (B220) MicroBeads or CD19 MicroBeads.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Positive selection of CD5+ cells from a mouse spleen cell suspension using CD5 (Ly-1) MicroBeads, a MiniMACS™ Separator, and an MS Column.
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Details
Products
CD5 (Ly-1) MicroBeads, mouse
- for 2×109 total cells
Download datasheet
130-049-301
Qty.:
 

Related products
CD45R (B220) MicroBeads, mouse (#130-049-501)
CD19 MicroBeads, mouse (#130-052-201)
References
1. Assenmacher et al. (1998) Eur. J. Immunol. 28: 1534–1543.
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