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| Pan T Cell Isolation Kit II |
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| Description |
| The Pan T Cell Isolation Kit II was developed for the isolation of untouched mouse T cells from single-cell suspensions from spleen or lymph nodes. |
| Details |
Background information
The kit is based on a cocktail of biotin-conjugated antibodies against CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, and Ter-119. The isolation of T cells is achieved by depletion of magnetically labeled cells.
Downstream applications
Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.
The Pan T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments1,2, T cell proliferation assays3,4 , Coculture with dendritic cells5 and Cytokine analysis6 experiments. |
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| Figure 1 |
| A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. T cells were isolated from this single-cell suspension using the Pan T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD90.2 T Cell Cocktail, mouse as well as with CD3ε-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| A: Before separation |
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| B: Isolated T cells |
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| Details |
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| Products |
| Pan T Cell Isolation Kit II, mouse |
- for 109 total cells
Download datasheet
130-095-130
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| References |
| 1. Felio et al. (2009) J. Exp. Med. 206: 2497–2509. |
| 2. Tasso et al. (2009) Carcinogenesis 30: 150–157. |
| 3. Kawaguchi et al. (2009) Blood 114: 2961–2968. |
| 4. Walters et al. (2009) J. Immunol. 182: 793–801. |
| 5. Alzabi et al. (2009) J. Immunol. 182: 6187–6194. |
| 6. Bruns et al. (2009) J. Immunol. 182:1314–1324. |
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