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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  Stem and progenitor cells
  Neural cells
  T cells
 
 
 
 
 
  NK cells
  B cells
  Monocytes and macrophages
  Myeloid derived suppressor cells
  Dendritic cells
  Antigen-presenting cells
  Granulocytes
  Leukocytes
  Endothelial cells
  Erythroid cells
  Cytokine-producing cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD3ε MicroBead Kit
Description
The CD3ε MicroBead Kit was developed for depletion of mouse T cells from single-cell supensions from spleen, lymph node, and thymus.
Details
Background information
CD3ε is a 20 kDa transmembrane protein and subunit of the TCR complex. Along with the γ and δ subunits of CD3, the ε chain is required for assembly and surface expression of the TCR complex. CD3 is expressed on thymocytes, mature T lymphocytes, and natural killer T (NKT) cells. With the CD3ε MicroBead Kit, mouse T lymphocytes and NKT cells can be depleted from various tissues including spleen, thymus, or lymph nodes.
Columns
LD or autoMACS Columns.
 
Figure 1
A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD3ε cells were depleted from this suspension using the CD3ε MicroBead Kit, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD45R (B220)-FITC and Anti-Biotin-PE, and analyzed using the MACSQuant® Analyzer.
A: Before separation
B: CD3ε- cells
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Details
Products
CD3ε MicroBead Kit, mouse
- for 109 total cells
Download datasheet
130-094-973
Qty.:
 

Related products
CD45R (B220) antibodies
Anti-Biotin antibodies
Miltenyi Biotec
Miltenyi Biotec
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