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| Ova Antigen Delivery Reagent |
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| Description |
Antigen targeting to antigen-presenting cells (APCs) via specific receptors has been used to induce effective antigen-specific T cell responses.1–5 The Ova Antigen Delivery Reagent has been developed for the in vitro targeting of ovalbumin to APCs, analysis of antigen uptake, and detection of antigen routes during antigen processing. It is a monoclonal anti-biotin antibody conjugated to ovalbumin and FITC. In combination with an appropriate biotinylated anti-receptor antibody any desired antigen uptake receptor can be targeted. This allows the functional characterization of new receptors on APCs for comparison with well-characterized ones, such as CD205 (DEC205) or DCIR2 (33D1). The Ova Antigen Delivery Reagent is also well suited for the analysis of antigen uptake and trafficking by fluorescent or confocal laser scanning microscopy.
The Antigen Delivery Module Set comprises all the reagents that are required for the isolation of DCs, antigen delivery, and subsequent analysis of antigen presentation.
Ova Antigen Delivery Reagent:
- Efficient targeting of ovalbumin to APCs
- Applicable to any desired antigen uptake receptor
- Reproducible results
- Easy-to-standardize procedure
- Comprehensive protocol
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| Applications |
- Analysis of antigen uptake by antigen-presenting cells, i.e., DCs, macrophages, and B cells.
- Analysis of antigen processing and intracellular trafficking of endocytic receptors.
- Research on cross-presentation
- Research on protocols of antigen delivery for DC vaccination.
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| Figure 1 |
| Proliferation of CD4+ T cells detected by CFSE labeling. CD4+ T cells from DO11.10 mice were cultured with DCs that were previously isolated using CD11c MicroBeads. Ovalbumin was targeted to DCs via Dectin-1, CD205 (DEC205), or DCIR2 (33D1), using biotinylated antibodies against the respective antigen uptake receptors in combination with the Ova Antigen Delivery Reagent. Cells were cultured in the presence of Pam3CysSK4 that induces DC maturation via toll-like receptors. In the absence of Ova Antigen Delivery Reagent no T cell proliferation was detected. |
| Dectin-1 |
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| CD205 (DEC205) |
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| DCIR2 (33D1) |
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| control |
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| References |
| 1. Bonifaz et al. (2002) J. Exp. Med. 196: 1627–1638. |
| 2. Dudziak et al. (2007) Science 315: 107–111. |
| 3. Mouriès et al. (2008) Blood 112: 3713–3722. |
| 4. Caminschi et al. (2008) Blood 112: 3264–3273. |
| 5. Sancho et al. (2008) J. Clin. Invest. 118: 2098–2110. |
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