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 - Reliable downstream analysis: organelle integrity maintained for full functionality
- Efficient: outstanding yield of isolated mitochondria
- Fast: pure mitochondria in less than two hours
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| Overview |
| The Mitochondria Isolation Kits allow for isolation of intact, viable mitochondria from human cells and tissue or mouse tissue in less than 2 hours. The protocol is based on the renowned MACS® Technology that combines high yield, purity and integrity of the isolated mitochondria. After cell lysis, the translocase of the outer mitochondrial membrane 22 (TOM22) is targeted by Anti-TOM22 MicroBeads to magnetically isolate functional human or mouse mitochondria. For details on the basic protocol please refer to figure 1. |
| Details |
In addition to the kit the equipment to get started consists of either a MidiMACS (one position magnet) or a QuadroMACS (four positions magnet) Separator and a MACS MultiStand. LS Columns sufficient for total 25 isolations are already included in every kit. The Mitochondria Isolation Kit, mouse tissue contains furthermore 25 Pre-Separation Filters, 30 μm for optimal processing of tissue samples.
After isolation the mitochondria can be further analyzed by Western blotting1,6 or in downstream functional asays i.e O2, membrane potential, ATP measurement4-5,7 or respiratory control4. Growing evidence also suggests that isolated mitochondria are well suited for mitochondrial RNA expression profiling studies2,3. |
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| Figure 1 |
| Principle of MACS Technology for isolation of human mitochondria. |
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| Favorites / Prices |
Request prices or add products to your favorite list.
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| Details |
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| Products |
| Mitochondria Isolation Kit, human |
- For 25 separations, each with up to 107 cells or appropriate amount of tissue.
Components
- 1.25 mL Anti-TOM22 MicroBeads, human
- 25 mL Lysis Buffer
- 56.25 mL 10x Separation Buffer
- 27.5 mL Storage Buffer
- 25 x LS Columns
Download datasheet
130-094-532
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| Mitochondria MidiMACS Starting Kit, human |
- For 25 separations, each with up to 107 cells or appropriate amount of tissue.
Components
- 1 x Mitochondria Isolation Kit, human
- 1 x MidiMACS Separator
- 1 x MACS MultiStand
Download datasheet
130-094-872
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| Mitochondria QuadroMACS Starting Kit, human |
- For 25 separations, each with up to 107 cells or appropriate amount of tissue.
Components
- 1 x Mitochondria Isolation Kit, human
- 1 x QuadroMACS Separator
- 1 x MACS MultiStand
Download datasheet
130-094-833
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| Mitochondria Isolation Kit, mouse tissue |
- For 25 separations, each with 50-100 mg tissue or with up to 107 cells.
Components
- 1.25 mL Anti-TOM22 MicroBeads, mouse
- 25 mL Lysis Buffer
- 56.25 mL 10x Separation Buffer
- 27.5 mL Storage Buffer
- 25 LS Columns
- 25 Pre-Separation Filters, 30 μm
Download datasheet
130-096-946
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| Mitochondria MidiMACS Starting Kit, mouse tissue |
- For 25 separations, each with 50-100 mg tissue or with up to 107 cells.
Components
- 1 Mitochondria Isolation Kit, mouse tissue
- 1 MidiMACS Separator
- 1 MACS MultiStand
Download datasheet
130-097-039
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| Mitochondria QuadroMACS Starting Kit, mouse tissue |
- For 25 separations, each with 50-100 mg tissue or with up to 107 cells.
Components
- 1 Mitochondria Isolation Kit, mouse tissue
- 1 QuadroMACS Separator
- 1 MACS MultiStand
Download datasheet
130-097-040
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| References |
| 1. Sacchi et al. (2011) Mol. Cell. Neurosc. 48(1): 20-28. |
| 2. Bandiera et al. (2011) PLoS ONE 6(6): e20746. |
| 3. Barrey et al. (2011) PLoS ONE 6(5): e20220. |
| 4. Minet and Gaster (2011) Biochem. Biophys. Res. Comm. 409(4): 591-595. |
| 5. Eriksen et al. (2011) J. Clin. Endocrinol. Metab. 96(8): E1298-1302. |
| 6. Guo et al. (2010) Mol. Cell. Proteomics 9(12): 2629-2641. |
| 7. Minet and Gaster (2010) Biochem. Biophys. Res. Comm. 402(1): 70-74. |
| 8. Hornig-Do et al. (2009) Anal. Biochem. 389(1): 1-5. |
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