| Description |
| The CD8+ T Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched CD8+ T cells from rhesus monkey PBMCs. Non-CD8+ T cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Highly pure untouched CD8+ T cells are obtained by depletion of the magnetically labeled cells. |
| Applications |
T cells isolated with the CD8+ T Cell Isolation Kit are used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on cytotoxic T cell activation.
Untouched CD8+ T cells may also be used for further isolation of specific cytotoxic T cell subsets. |
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| Figure 1 |
| Isolation of untouched CD8+ T cells from rhesus monkey PBMCs using the CD8+ T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD8-PE, and CD3-APC. Plots show viable CD45+ leukocytes. |
| A: PBMCs before separation |
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| B: Isolated CD8+ T cells |
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