|
|
 |
| NK Cell Activation/Expansion Kit, human |
|
| Description |
| The NK Cell Activation/Expansion Kit is designed to activate and expand human NK cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against human CD335 (NKp46) and CD2. Anti-Biotin MACSiBead Particles loaded with biotinylated antibodies are used to activate and expand resting NK cells purified from human blood or PBMCs. |
| Details |
Downstream applications
NK cells, expanded by using antibody-loaded MACSiBead Particles, can be used for many downstream applications, for example, cytokine analysis, cytolytic activity, gene expression analysis, or functional studies.1 |
| |
| Figure 1 |
| Anti-Biotin MACSiBead Particles were loaded with CD335 (NKp46) and CD2 antibodies. NK cells were isolated using the NK Cell Isolation Kit, human, and expanded using one loaded Anti-Biotin MACSiBead Particle per two NK cells. NK cells were cultured in medium supplemented with 10% FCS and 500 IU/mL rIL-2 at an initial density of 106 NK cells per mL. Cells were expanded for 3 weeks. For comparison, NK cells were cultured in medium supplemented with 10% FCS and 500 IU/mL rIL-2 alone. |
 |
|
| Figure 2 |
| Purified human NK cells were activated and expanded using the NK Cell Activation/Expansion Kit. After 2 weeks of culture in medium supplemented with 10% FCS and 500 IU/mL rIL-2, cytotoxicity of NK cells was determined by incubation with K562 target cells in different ratios. In addition, cells incubated in medium supplemented with 10% FCS and 500 IU/mL rIL-2 alone and freshly isolated NK cells were analyzed. Specific target cell lysis by NK cells was determined after 4 hours. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| NK Cell Activation/Expansion Kit, human |
Components
- 2 mL Anti-Biotin MACSiBead™ Particles, cell culture grade
- 0.4 mL CD2-Biotin, human – functional grade
- 0.4 mL CD335 (NKp46)-Biotin, human – functional grade
Download datasheet
130-094-483
|
|
| References |
| 1. Reim et al.(2009) Cancer Res. 69:8058-8066. |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
