Myeloid-Derived Suppressor Cell Isolation Kit
Description Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells with a remarkable capacity to induce T cell dysfunction. Phenotypically characterized as CD11b+Gr-1+–expressing cells, partial blocking of the cell differentiation of immature myeloid progenitor cells into granulocytes, macrophages, or dendritic cells results in the expansion of this population in various pathological conditions (cancer, infection, autoimmune diseases).The Myeloid-Derived Suppressor Cell Isolation Kit has been developed for the isolation of Gr-1highLy-6G+ and/or Gr-1dimLy-6G– myeloid cells. Gr-1dimLy-6G– cells can be further subdivided according to the expression of the marker Ly-6C into Gr-1dimLy-6ChighLy-6G– and Gr-1dimLy-6ClowLy-6G– cells. The proportion between Ly-6Chigh and Ly-6Clow subpopulations depends on the used tumor models. Applications Isolation of MDSCs for the analysis of their functional properties, e.g., on T cell activation and effector functions in different experimental mouse models. Isolation of MDSCs for phenotypical analysis. Isolation of MDSCs for downstream analysis such as gene expression profiling. Columns For the first magnetic separation: LS or autoMACS® Columns; for second magnetic separation: MS or autoMACSTM Columns.		Figure 1 Myeloid-derived suppressor cells were isolated from a spleen of a BALB/c mouse using the Myeloid-Derived Suppressor Cell Isolation Kit, two LS Columns, two MS Columns, and an appropriate MACS Separator. Cells were fluorescently stained with CD11b-APC and Anti-Gr-1-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Spleen cells before separation Enriched Gr-1highLy-6G+ cells Enriched Gr-1dimLy-6G– cells Figure 2 Myeloid-derived suppressor cells were isolated from a spleen of a BALB/c mouse using the Myeloid-Derived Suppressor Cell Isolation Kit, two LS Columns, two MS Columns, and an appropriate MACS Separator. Cells were fluorescently stained with CD11b-APC and Anti-Ly-6G-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Spleen cells before separation Enriched Gr-1highLy-6G+ cells Enriched Gr-1dimLy-6G– cells

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