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| MACS® GMP PepTivator® EBV BZLF1 |
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| Overview |
| MACS® GMP PepTivator® EBV BZLF1 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acids overlap. It has been developed for efficient in vitro stimulation and subsequent isolation of EBV BZLF1–specific CD4+ and CD8+ T cells. |
| Details |
Background information
Epstein-Barr virus (EBV) is a human γ-herpesvirus with B cell growth–transforming ability and carcinogenic potential. More than 90% of human adults are infected with EBV. In healthy individuals, EBV typically establishes a persistent latent infection, in which the virus can be detected in resting, non-proliferating peripheral B lymphocytes.
BZLF1 is a transcriptional activator and regulates a promoter of an EBV gene. It plays a key role in the switch from latent to productive EBV infection.
Applications
T cells are essential for the control of the outgrowth of EBV-infected B cells. BZLF1-specific CD4+ and CD8+ T cells have been found in EBV-infected individuals.1–4
Adoptive transfer of BZLF1-specific T cells may be an effective tool for the treatment of EBV-associated malignancies.
In vitro stimulation of BZLF1-specific CD4+ and CD8+ T cells with MACS GMP PepTivator EBV BZLF1 causes the production of the effector cytokine IFN-γ. The secretion of IFN-γ then permits the enrichment of BZLF1-specific effector/memory T cells using the CliniMACS® Cytokine Capture System (IFN-gamma).
Quality statement
MACS GMP Products are manufactured and tested under a certified ISO 9001 quality system and in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
No animal-, human-, or virus-derived materials were used for manufacture of these products. |
| Disclaimer |
| MACS GMP Products are for research use and ex vivo cell culture processing only, and are not intended for human in vivo applications. |
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| Details |
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| Products |
| MACS GMP PepTivator EBV BZLF1 |
| Availability: Worldwide (1) |
- 60 nmol/peptide for the stimulation of 109 cells
Download datasheet
170-076-130
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| (1) For availability in your country please contact your local representative. |
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| References |
| 1. Bogedain et al. (1995) J. Virol. 69: 4872–4879. |
| 2. Saulquin et al. (2000) Eur. J. Immunol. 30: 2531–2539. |
| 3. Precopio et al. (2003) J. Immunol. 170: 2590–2598. |
| 4. Tynan et al. (2005) J. Exp. Med. 202: 1249–1260. |
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